Bee Mines are a Fight Item that can be made with 1 Board, 4 Bees and 1 Flint. A Science Machine is required to prototype a Bee Mine. Once placed, the mine will explode when something comes near it, spawning 4 regular Bees aggressive to whomever/whatever triggered the mine.
Bees from a triggered mine will not target the player, since they conform to regular Bee behaviour. That being the case, they will fall asleep if released at night or in Caves.

Hammering the bee mine will cause the player to exclaim "BEEEEES!" and they will be attacked as though they had triggered the mine.

Note that if the Bees from the Mine are attacked by their target, other Bees from either Bee Boxes or Bee Hives will come to help. Once the target is dead the Bees will remain and can be caught to make another mine. Remember however that this counts as aggression and bees don't discriminate. If there were others nearby assisting against the bomb target, those will attack the player too when using the Bug Net.